by Jon Fleetwood,Jon Fleetwood:
Do hantavirus PCR tests mistake human DNA for a virus?
A BLAST analysis of the forward primer, reverse primer, and fluorescent probe used in a published hantavirus RT-qPCR assay revealed repeated exact matches to human genomic material, raising questions about whether human nucleic acids could plausibly contribute to positive hantavirus PCR signal generation under some assay conditions.
BLAST stands forBasic Local Alignment Search Tool, a widely used bioinformatics algorithm for comparing biological sequences.
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In plain English, portions of the genetic sequences used by the PCR test to supposedly detect hantavirus also directly match human DNA sequences.
That means the test components were not exclusively unique to hantavirus at the sequence level.
Positive results could indicate the presence of human material, not viral.
The revelation comes as the mainstream media israising alarmover an alleged hantavirus outbreak on a cruise ship off West Africa.
The new BLAST findings become more significant when viewed alongside the originalU.S. Patent 5,210,015, which explicitly states that PCR probe complementarity “need not be perfect” and that a detectable signal can occur through “polymerization-independent cleavage,” meaning detectable fluorescent signal generation does not necessarily require full target amplification.
Source: SGT Report
